LOCUS pCM62-Prha-T7SL- 8089 bp ds-DNA circular 23-DEC-2011 ACCESSION pCM62-Prha-T7SL- VERSION pCM62-Prha-T7SL- KEYWORDS . FEATURES Location/Qualifiers misc_feature 3334..3336 /label="alanine 250" /vntifkey="21" rep_origin 948..1656 /label="Rep_Origin_1" /vntifkey="33" /note="oriV; IncP origin of replication" gene 5271..5936 /label="tetR" /vntifkey="60" gene complement(3966..5165) /label="tetA" /gene="tetA" /vntifkey="60" gene 2587..3735 /label="trfA" /vntifkey="60" oriT 1667..2405 /label="oriT_1" /vntifkey="104" /note="IncP origin of transfer" gene 1944..2060 /label="traJ'" /vntifkey="60" CDS 1944..2060 /label="traJ'" /gene="traJ'" /codon_start="1" /transl_table="11" /product="TraJ'" /protein_id="AAK73400.1" /vntifkey="4" /note="truncated oriT-recognizing protein" /db_xref="GI:14794590.0" misc_feature 795..923 /label="dbl term" /vntifkey="21" CDS 93..770 /label="RFP" /vntifkey="4" terminator 5973..6078 /label="T0" /vntifkey="43" promoter 7855..91 /label="PrhaB" /vntifkey="30" CDS 7021..7854 /label="rhaS" /vntifkey="4" CDS 6099..7118 /label="rhaR" /vntifkey="4" misc_feature 28..66 /label="T7 stem loop" /vntifkey="21" misc_feature 29 /label="trascriptional start?" /vntifkey="21" ORIGIN 1 gaattcaggc gctttttaga ctggtcgtag ggagaccaca acggtttccc tctagaaata 61 attttgagat cttttaagaa ggagatatac atatggcgag tagcgaagac gttatcaaag 121 agttcatgcg tttcaaagtt cgtatggaag gttccgttaa cggtcacgag ttcgaaatcg 181 aaggtgaagg tgaaggtcgt ccgtacgaag gtacccagac cgctaaactg aaagttacca 241 aaggtggtcc gctgccgttc gcttgggaca tcctgtcccc gcagttccag tacggttcca 301 aagcttacgt taaacacccg gctgacatcc cggactacct gaaactgtcc ttcccggaag 361 gtttcaaatg ggaacgtgtt atgaacttcg aagacggtgg tgttgttacc gttacccagg 421 actcctccct gcaagacggt gagttcatct acaaagttaa actgcgtggt accaacttcc 481 cgtccgacgg tccggttatg cagaaaaaaa ccatgggttg ggaagcttcc accgaacgta 541 tgtacccgga agacggtgct ctgaaaggtg aaatcaaaat gcgtctgaaa ctgaaagacg 601 gtggtcacta cgacgctgaa gttaaaacca cctacatggc taaaaaaccg gttcagctgc 661 cgggtgctta caaaaccgac atcaaactgg acatcacctc ccacaacgaa gactacacca 721 tcgttgaaca gtacgaacgt gctgaaggtc gtcactccac cggtgcttaa ggatccaaac 781 tcgagtaagg atctccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt 841 tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc 901 gggtgggcct ttctgcgttt atacctaggc agaagtggtc agcttggctg ccatttttgg 961 ggtgaggccg ttcgcggccg aggggcgcag cccctggggg gatgggaggc ccgcgttagc 1021 gggccgggag ggttcgagaa gggggggcac cccccttcgg cgtgcgcggt cacgcgcaca 1081 gggcgcagcc ctggttaaaa acaaggttta taaatattgg tttaaaagca ggttaaaaga 1141 caggttagcg gtggccgaaa aacgggcgga aacccttgca aatgctggat tttctgcctg 1201 tggacagccc ctcaaatgtc aataggtgcg cccctcatct gtcagcactc tgcccctcaa 1261 gtgtcaagga tcgcgcccct catctgtcag tagtcgcgcc cctcaagtgt caataccgca 1321 gggcacttat ccccaggctt gtccacatca tctgtgggaa actcgcgtaa aatcaggcgt 1381 tttcgccgat ttgcgaggct ggccagctcc acgtcgccgg ccgaaatcga gcctgcccct 1441 catctgtcaa cgccgcgccg ggtgagtcgg cccctcaagt gtcaacgtcc gcccctcatc 1501 tgtcagtgag ggccaagttt tccgcgaggt atccacaacg ccggcggccg cggtgtctcg 1561 cacacggctt cgacggcgtt tctggcgcgt ttgcagggcc atagacggcc gccagcccag 1621 cggcgagggc aaccagcccg gtgagcgtcg gaaagggtcg acggatcttt tccgctgcat 1681 aaccctgctt cggggtcatt atagcgattt tttcggtata tccatccttt ttcgcacgat 1741 atacaggatt ttgccaaagg gttcgtgtag actttccttg gtgtatccaa cggcgtcagc 1801 cgggcaggat aggtgaagta ggcccacccg cgagcgggtg ttccttcttc actgtccctt 1861 attcgcacct ggcggtgctc aacgggaatc ctgctctgcg aggctggccg gctaccgccg 1921 gcgtaacaga tgagggcaag cggatggctg atgaaaccaa gccaaccagg aagggcagcc 1981 cacctatcaa ggtgtactgc cttccagacg aacgaagagc gattgaggaa aaggcggcgg 2041 cggccggcat gagcctgtag gcctacctgc tggccgtcgg ccagggctac aaaatcacgg 2101 gcgtcgtgga ctatgagcac gtccgcgagc tggcccgcat caatggcgac ctgggccgcc 2161 tgggcggcct gctgaaactc tggctcaccg acgacccgcg cacggcgcgg ttcggtgatg 2221 ccacgatcct cgccctgctg gcgaagatcg aagagaagca ggacgagctt ggcaaggtca 2281 tgatgggcgt ggtccgcccg agggcagagc catgactttt ttagccgcta aaacggccgg 2341 ggggtgcgcg tgattgccaa gcacgtcccc atgcgctcca tcaagaagag cgacttcgcg 2401 gagctggtat tcgtgcaggg caagattcgg aataccaagt acgagaagga cggccagacg 2461 gtctacggga ccgacttcat tgccgataag gtggattatc tggacaccaa ggcaccaggc 2521 gggtcaaatc aggaataagg gcacattgcc ccggcgtgag tcggggcaat cccgcaagga 2581 gggtgaatga atcggacgtt tgaccggaag gcatacaggc aagaactgat cgacgcgggg 2641 ttttccgccg aggatgccga aaccatcgca agccgcaccg tcatgcgtgc gccccgcgaa 2701 accttccagt ccgtcggctc gatggtccag caagctacgg ccaagatcga gcgcgacagc 2761 gtgcaactgg ctccccctgc cctgcccgcg ccatcggccg ccgtggagcg ttcgcgtcgt 2821 ctcgaacagg aggcggcagg tttggcgaag tcgatgacca tcgacacgcg aggaactatg 2881 acgaccaaga agcgaaaaac cgccggcgag gacctggcaa aacaggtcag cgaggccaag 2941 caggccgcgt tgctgaaaca cacgaagcag cagatcaagg aaatgcagct ttccttgttc 3001 gatattgcgc cgtggccgga cacgatgcga gcgatgccaa acgacacggc ccgctctgcc 3061 ctgttcacca cgcgcaacaa gaaaatcccg cgcgaggcgc tgcaaaacaa ggtcattttc 3121 cacgtcaaca aggacgtgaa gatcacctac accggcgtcg agctgcgggc cgacgatgac 3181 gaactggtgt ggcagcaggt gttggagtac gcgaagcgca cccctatcgg cgagccgatc 3241 accttcacgt tctacgagct ttgccaggac ctgggctggt cgatcaatgg ccggtattac 3301 acgaaggccg aggaatgcct gtcgcgccta caggcgacgg cgatgggctt cacgtccgac 3361 cgcgttgggc acctggaatc ggtgtcgctg ctgcaccgct tccgcgtcct ggaccgtggc 3421 aagaaaacgt cccgttgcca ggtcctgatc gacgaggaaa tcgtcgtgct gtttgctggc 3481 gaccactaca cgaaattcat atgggagaag taccgcaagc tgtcgccgac ggcccgacgg 3541 atgttcgact atttcagctc gcaccgggag ccgtacccgc tcaagctgga aaccttccgc 3601 ctcatgtgcg gatcggattc cacccgcgtg aagaagtggc gcgagcaggt cggcgaagcc 3661 tgcgaagagt tgcgaggcag cggcctggtg gaacacgcct gggtcaatga tgacctggtg 3721 cattgcaaac gctagggcct tgtggggtca gttccggctg ggggttcagc agccagcgct 3781 ttactggcat ttcaggaaca agcgggcact gctcgacgca cttgcttcgc tcagtatcgc 3841 tcgggacgca cggcgcgctc tacgaactgc cgataaacag aggattaaaa ttgacaattc 3901 tagggcgcgt atagcttgcc ggaagtcgcc ttgacccgca tggcataggc ctatcgtttc 3961 cacgatcagc gatcggctcg ttgccctgcg ccgctccaaa gcccgcgacg cagcgccggc 4021 aggcagagca agtagagggc agcgcctgca atccatgccc acccgttcca cgttgttata 4081 gaagccgcat agatcgccgt gaagaggagg ggtccgacga tcgaggtcag gctggtgagc 4141 gccgccagtg agccttgcag ctgcccctga cgttcctcat ccacctgcct ggacaacatt 4201 gcttgcagcg ccggcattcc gatgccaccc gaagcaagca ggaccatgat cgggaacgcc 4261 atccatcccc gtgtcgcgaa ggcaagcagg atgtagcctg tgccgtcggc aatcattccg 4321 agcatgagtg cccgcctttc gccgagccgg gcggctacag ggccggtgat cattgcctgg 4381 gcgagtgaat gcagaatgcc aaatgcggca agcgaaatgc cgatcgtggt cgcgtcccag 4441 tgaaagcgat cctcgccgaa aatgacccaa agcgcggccg gcacctgtcc gacaagttgc 4501 atgatgaaga agaccgccat cagggcggcg acgacggtca tgccccgggc ccaccggaac 4561 gaagcgagcg ggttgagagc ctcccggcgt aacggccggc gttcgccttt gtgcgactcc 4621 ggcaaaagga aacagcccgt caggaaattg aggccgttca aggctgccgc ggcgaagaac 4681 ggagcgtggg gggagaaacc gcccatcagc ccaccgagca caggtcccgc gaccatcccg 4741 aacccgaaac aggcgctcat gaagccgaag tgccgcgcgc gctcatcgcc atcagtgata 4801 tcggcaatat aagcgccggc taccgcccca gtcgccccgg tgatgccggc cacgatccgc 4861 ccgatataga gaacccaaag gaaaggcgct gtcgccatga tggcgtagtc gacagtggcg 4921 ccggccagcg agacgagcaa gattggccgc cgcccgaaac gatccgacag cgcgcccagc 4981 acaggtgcgc aggcaaattg caccaacgca tacagcgcca gcagaatgcc atagtgggcg 5041 gtgacgtcgt tcgagtgaac cagatcgcgc aggaggcccg gcagcaccgg cataatcagg 5101 ccgatgccga cagcgtcgag cgcgacagtg ctcagaatta cgatcagggg tatgttgggt 5161 ttcacgtctg gcctccggac cagcctccgc tggtccgatt gaacgcgcgg attctttatc 5221 actgataagt tggtggacat attatgttta tcagtgataa agtgtcaatc atgacaaagt 5281 tgcagccgaa tacagtgatc cgtgccgccc tggacctgtt gaacgaggtc ggcgtagacg 5341 gtctgacgac acgcaaactg gcggaacggt tgggggttca gcagccggcg ctttactggc 5401 acttcaggaa caagcgggcg ctgctcgacg cactggccga agccatgctg gcggagaatc 5461 atacgcattc ggtgccgaga gccgacgacg actggcgctc atttctgatc gggaatgccc 5521 gcagcttcag gcaggcgctg ctcgcctacc gcgatggcgc gcgcatccat gccggcacgc 5581 gaccgggcgc accgcagatg gaaacggccg acgcgcagct tcgcttcctc tgcgaggcgg 5641 gtttttcggc cggggacgcc gtcaatgcgc tgatgacaat cagctacttc actgttgggg 5701 ccgtgcttga ggagcaggcc ggcgacagcg atgccggcga gcgcggcggc accgttgaac 5761 aggctccgct ctcgccgctg ttgcgggccg cgatagacgc cttcgacgaa gccggtccgg 5821 acgcagcgtt cgagcaggga ctcgcggtga ttgtcgatgg attggcgaaa aggaggctcg 5881 ttgtcaggaa cgttgaagga ccgagaaagg gtgctaagaa accattatta tcatgacatt 5941 aacctataaa aataggcgta tcacgaacta gtgcttggat tctcaccaat aaaaaacgcc 6001 cggcggcaac cgagcgttct gaacaaatcc agatggagtt ctgaggtcat tactggatct 6061 atcaacagga gtccaagcga gctcggcatg gcgaattaat ctttctgcga attgagatga 6121 cgccactggc tgggcgtcat cccggtttcc cgggtaaaca ccaccgaaaa atagttacta 6181 tcttcaaagc cacattcggt cgaaatatca ctgattaaca ggcggctatg ctggagaaga 6241 tattgcgcat gacacactct gacctgtcgc agatattgat tgatggtcat tccagtctgc 6301 tggcgaaatt gctgacgcaa aacgcgctca ctgcacgatg cctcatcaca aaatttatcc 6361 agcgcaaagg gacttttcag gctagccgcc agccgggtaa tcagcttatc cagcaacgtt 6421 tcgctggatg ttggcggcaa cgaatcactg gtgtaacgat ggcgattcag caacatcacc 6481 aactgcccga acagcaactc agccatttcg ttagcaaacg gcacatgctg actactttca 6541 tgctcaagct gaccgataac ctgccgcgcc tgcgccatcc ccatgctacc taagcgccag 6601 tgtggttgcc ctgcgctggc gttaaatccc ggaatcgccc cctgccagtc aagattcagc 6661 ttcagacgct ccgggcaata aataatattc tgcaaaacca gatcgttaac ggaagcgtag 6721 gagtgtttat cgtcagcatg aatgtaaaag agatcgccac gggtaatgcg ataagggcga 6781 tcgttgagta catgcaggcc attaccgcgc cagacaatca ccagctcaca aaaatcatgt 6841 gtatgttcag caaagacatc ttgcggataa cggtcagcca cagcgactgc ctgctggtcg 6901 ctggcaaaaa aatcatcttt gagaagtttt aactgatgcg ccaccgtggc tacctcggcc 6961 agagaacgaa gttgattatt cgcaatatgg cgtacaaata cgttgagaag attcgcgtta 7021 ttgcagaaag ccatcccgtc cctggcgaat atcacgcggt gaccagttaa actctcggcg 7081 aaaaagcgtc gaaaagtggt tactgtcgct gaatccacag cgataggcga tgtcagtaac 7141 gctggcctcg ctgtggcgta gcagatgtcg ggctttcatc agtcgcaggc ggttcaggta 7201 tcgctgaggc gtcagtcccg tttgctgctt aagctgccga tgtagcgtac gcagtgaaag 7261 agaaaattga tccgccacgg catcccaatt cacctcatcg gcaaaatggt cctccagcca 7321 ggccagaagc aagttgagac gtgatgcgct gttttccagg ttctcctgca aactgctttt 7381 acgcagcaag agcagtaatt gcataaacaa gatctcgcga ctggcggtcg agggtaaatc 7441 attttcccct tcctgctgtt ccatctgtgc aaccagctgt cgcacctgct gcaatacgct 7501 gtggttaacg cgccagtgag acggatactg cccatccagc tcttgtggca gcaactgatt 7561 cagcccggcg agaaactgaa atcgatccgg cgagcgatac agcacattgg tcagacacag 7621 attatcggta tgttcataca gatgccgatc atgatcgcgt acgaaacaga ccgtgccacc 7681 ggtgatggta tagggctgcc cattaaacac atgaataccc gtgccatgtt cgacaatcac 7741 aatttcatga aaatcatgat gatgttcagg aaaatccgcc tgcgggagcc ggggttctat 7801 cgccacggac gcgttaccag acggaaaaaa atccacacta tgtaatacgg tcatactggc 7861 ctcctgatgt cgtcaacacg gcgaaatagt aatcacgagg tcaggttctt accttaaatt 7921 ttcgacggaa aaccacgtaa aaaacgtcga tttttcaaga tacagcgtga attttcagga 7981 aatgcggtga gcatcacatc accacaattc agcaaattgt gaacatcatc acgttcatct 8041 ttccctggtt gccaatggcc cattttcctg tcagtaacga gaaggtcgc //