LOCUS       pGFPuv_cterm_signal     3338 bp    DNA     circular SYN 26-OCT-2009
DEFINITION  Cloning vector pGFPuv, complete sequence.
ACCESSION   U62636
VERSION     U62636.1  GI:1490528
SOURCE      Cloning vector pGFPuv.
  ORGANISM  Cloning vector pGFPuv
FEATURES             Location/Qualifiers
     gene            1538..2398
                     /gene="bla"
                     /vntifkey="60"
     CDS             1538..2398
                     /gene="bla"
                     /function="confers resistance to ampicillin"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase"
                     /protein_id="AAB06049.1"
                     /db_xref="GI:1490530"
                     /vntifkey="4"
                     /label=bla
     misc_feature    2546..3189
                     /vntifkey="21"
                     /label=pUC\ori
     misc_feature    95..178
                     /vntifkey="21"
                     /label=Plac
     misc_feature    179..199
                     /vntifkey="21"
                     /label=lac\operator
     CDS             289..1071
                     /vntifkey="4"
                     /label=GFPuv
     CDS             1006..1068
                     /vntifkey="4"
                     /label=signal_peptide
BASE COUNT      878 a       788 c       836 g       836 t 
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc 
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc 
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa 
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg 
      241 catgcctgca ggtcgactct agaggatccc cgggtaccgg tagaaaaaat gagtaaagga 
      301 gaagaacttt tcactggagt tgtcccaatt cttgttgaat tagatggtga tgttaatggg 
      361 cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa catacggaaa acttaccctt 
      421 aaatttattt gcactactgg aaaactacct gttccatggc caacacttgt cactactttc 
      481 tcttatggtg ttcaatgctt ttcccgttat ccggatcata tgaaacggca tgactttttc 
      541 aagagtgcca tgcccgaagg ttatgtacag gaacgcacta tatctttcaa agatgacggg 
      601 aactacaaga cgcgtgctga agtcaagttt gaaggtgata cccttgttaa tcgtatcgag 
      661 ttaaaaggta ttgattttaa agaagatgga aacattctcg gacacaaact cgagtacaac 
      721 tataactcac acaatgtata catcacggca gacaaacaaa agaatggaat caaagctaac 
      781 ttcaaaattc gccacaacat tgaagatgga tccgttcaac tagcagacca ttatcaacaa 
      841 aatactccaa ttggcgatgg ccctgtcctt ttaccagaca accattacct gtcgacacaa 
      901 tctgcccttt cgaaagatcc caacgaaaag cgtgaccaca tggtccttct tgagtttgta 
      961 actgctgctg ggattacaca tggcatggat gagctcggcg gcggcggcag caaggtctac 
     1021 ggcaaggaac agtttttgcg gatgcgccag agcatgttcc ccgatcgcta aactagtcgg 
     1081 ccgtacgggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg 
     1141 cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt 
     1201 cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag 
     1261 cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga 
     1321 aaataccgca tcaggcggcc ttaagggcct cgtgatacgc ctatttttat aggttaatgt 
     1381 catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac 
     1441 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc 
     1501 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt 
     1561 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct 
     1621 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga 
     1681 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag 
     1741 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca 
     1801 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga 
     1861 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag 
     1921 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc 
     1981 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa 
     2041 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt 
     2101 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg 
     2161 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt 
     2221 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg 
     2281 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat 
     2341 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact 
     2401 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa 
     2461 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt 
     2521 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt 
     2581 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg 
     2641 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca 
     2701 gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt 
     2761 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga 
     2821 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc 
     2881 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact 
     2941 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga 
     3001 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg 
     3061 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt 
     3121 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt 
     3181 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga 
     3241 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac 
     3301 gaccgagcgc agcgagtcag tgagcgagga agcggaag 
//