LOCUS pGG_00005 5687 bp dna circular UNK ACCESSION pJWT00001 KEYWORDS ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; . FEATURES Location/Qualifiers CDS 1..2 /vntifkey="4" /ApEinfo_fwdcolor=#00ffff /label=AmpR /ApEinfo_revcolor=#00ffff misc_marker 138..434 /vntifkey="22" /ApEinfo_fwdcolor=pink /label=Kan/neoR 5prime /ApEinfo_revcolor=pink /note="encodes nptII (aka AphA, neoR), gives kan and neo resistance, with native promoter in on AatII and SpeI from Lutz vector pZE2PLtetO-1 MCS2" CDS 483..1565 /vntifkey="4" /ApEinfo_fwdcolor=pink /label=lacI /ApEinfo_revcolor=pink /note="lacI does not contain internal EcoRV site" promoter 1934..2009 /vntifkey="30" /ApEinfo_fwdcolor=pink /label=lacUV5 promoter /ApEinfo_revcolor=pink misc_binding 1950..2013 /vntifkey="20" /ApEinfo_fwdcolor=pink /label=lac operator /ApEinfo_revcolor=pink RBS 2029..2048 /vntifkey="32" /ApEinfo_fwdcolor=pink /label=RBS /ApEinfo_revcolor=pink CDS 2049..2726 /vntifkey="4" /ApEinfo_fwdcolor=pink /label=RFP /ApEinfo_revcolor=pink terminator 2751..2784 /label=Bba_1000_Term CDS complement(2881..3414) /label=aacC1 misc_marker 3706..3967 /vntifkey="22" /ApEinfo_fwdcolor=pink /label=Kan/neoR 3prime /ApEinfo_revcolor=pink /note="encodes nptII (aka AphA, neoR), gives kan and neo resistance, with native promoter in on AatII and SpeI from Lutz vector pZE2PLtetO-1 MCS2" rep_origin 4011..4693 /vntifkey="33" /ApEinfo_fwdcolor=pink /label=colE1 /ApEinfo_revcolor=pink terminator 4699..4804 /vntifkey="43" /ApEinfo_fwdcolor=pink /label=T0 /ApEinfo_revcolor=pink CDS 4834..5687 /vntifkey="4" /ApEinfo_fwdcolor=#00ffff /label=AmpR /ApEinfo_revcolor=#00ffff primer_bind 4850..4869 /ApEinfo_fwdcolor=#996633 /label=RDR00097 /ApEinfo_revcolor=#996633 misc_binding 4969..4974 /ApEinfo_fwdcolor=#00ff00 /label=BsaI_site /ApEinfo_revcolor=#00ff00 variation 4970..4970 /ApEinfo_fwdcolor=#ff0000 /label=AtoG_mutation /ApEinfo_revcolor=#ff0000 ORIGIN 1 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga 61 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga 121 aaagtgccac ccccgggatg attgaacaag atggattgca cgcaggttct ccggccgctt 181 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg 241 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg 301 gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg 361 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg 421 gcgaagtgcc gggggacgtc ggtgcctaat gagtgagcta acttacatta attgcgttgc 481 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc 541 aacgcgcggg gagaggcggt ttgcgtattg ggcgccaggg tggtttttct tttcaccagt 601 gagacgggca acagctgatt gcccttcacc gcctggccct gagagagttg cagcaagcgg 661 tccacgctgg tttgccccag caggcgaaaa tcctgtttga tggtggttaa cggcgggata 721 taacatgagc tgtcttcggt atcgtcgtat cccactaccg agatgtccgc accaacgcgc 781 agcccggact cggtaatggc gcgcattgcg cccagcgcca tctgatcgtt ggcaaccagc 841 atcgcagtgg gaacgatgcc ctcattcagc atttgcatgg tttgttgaaa accggacatg 901 gcactccagt cgccttcccg ttccgctatc ggctgaattt gattgcgagt gagatattta 961 tgccagccag ccagacgcag acgcgccgag acagaactta atgggcccgc taacagcgcg 1021 atttgctggt gacccaatgc gaccagatgc tccacgccca gtcgcgtacc gtcttcatgg 1081 gagaaaataa tactgttgat gggtgtctgg tcagagacat caagaaataa cgccggaaca 1141 ttagtgcagg cagcttccac agcaatggca tcctggtcat ccagcggata gttaatgatc 1201 agcccactga cgcgttgcgc gagaagattg tgcaccgccg ctttacaggc ttcgacgccg 1261 cttcgttcta ccatcgacac caccacgctg gcacccagtt gatcggcgcg agatttaatc 1321 gccgcgacaa tttgcgacgg cgcgtgcagg gccagactgg aggtggcaac gccaatcagc 1381 aacgactgtt tgcccgccag ttgttgtgcc acgcggttgg gaatgtaatt cagctccgcc 1441 atcgccgctt ccactttttc ccgcgttttc gcagaaacgt ggctggcctg gttcaccacg 1501 cgggaaacgg tctgataaga gacaccggca tactctgcga catcgtataa cgttactggt 1561 ttcacattca ccaccctgaa ttgactctct tccgggcgct atcatgccat accgcgaaag 1621 gttttgcgcc attcgatggt gtccgggatc tcgacgctct cccttatgcg actcctgcat 1681 taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc 1741 atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc 1801 gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc 1861 gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc 1921 gtagaggatc gagatcgttt aggcacccca ggctttacac tttatgcttc cggctcgtat 1981 aatgtgtgga attgtgagcg gataacaatt tcagaattca aaagatcttt taagaaggag 2041 atatacatat ggcgagtagc gaagacgtta tcaaagagtt catgcgtttc aaagttcgta 2101 tggaaggttc cgttaacggt cacgagttcg aaatcgaagg tgaaggtgaa ggtcgtccgt 2161 acgaaggtac ccagaccgct aaactgaaag ttaccaaagg tggtccgctg ccgttcgctt 2221 gggacatcct gtccccgcag ttccagtacg gttccaaagc ttacgttaaa cacccggctg 2281 acatcccgga ctacctgaaa ctgtccttcc cggaaggttt caaatgggaa cgtgttatga 2341 acttcgaaga cggtggtgtt gttaccgtta cccaggactc ctccctgcaa gacggtgagt 2401 tcatctacaa agttaaactg cgtggtacca acttcccgtc cgacggtccg gttatgcaga 2461 aaaaaaccat gggttgggaa gcttccaccg aacgtatgta cccggaagac ggtgctctga 2521 aaggtgaaat caaaatgcgt ctgaaactga aagacggtgg tcactacgac gctgaagtta 2581 aaaccaccta catggctaaa aaaccggttc agctgccggg tgcttacaaa accgacatca 2641 aactggacat cacctcccac aacgaagact acaccatcgt tgaacagtac gaacgtgctg 2701 aaggtcgtca ctccaccggt gcttaaggat ccaaactcga gtaaggatct cgcaaaaaac 2761 cccgcttcgg cggggttttt tcgccctagg gcgttcggct gcggcgagcg gtatcagctc 2821 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt 2881 ttaggtggcg gtacttgggt cgatatcaaa gtgcatcact tcttcccgta tgcccaactt 2941 tgtatagaga gccactgcgg gatcgtcacc gtaatctgct tgcacgtaga tcacataagc 3001 accaagcgcg ttggcctcat gcttgaggag attgatgagc gcggtggcaa tgccctgcct 3061 ccggtgctcg ccggagactg cgagatcata gatatagatc tcactacgcg gctgctcaaa 3121 cttgggcaga acgtaagccg cgagagcgcc aacaaccgct tcttggtcga aggcagcaag 3181 cgcgatgaat gtcttactac ggagcaagtt cccgaggtaa tcggagtccg gctgatgttg 3241 ggagtaggtg gctacgtctc cgaactcacg accgaaaaga tcaagagcag cccgcatgga 3301 tttgacttgg tcagggccga gcctacatgt gcgaatgatg cccatacttg agccacctaa 3361 ctttgtttta gggcgactgc cctgctgcgt aacatcgttg ctgctgcgta acatcgttgc 3421 tgctccataa catcaaacat cgacccacgg cgtaacgcgc ttgctgcttg gatgcccgag 3481 gcatagactg tacaaaaaaa cagtcataac aagccatgaa aaccgccact gcgccgttac 3541 caccgctgcg ttcggtcaag gttctggacc agttgcgtga gcgcatacgc tacttgcatt 3601 acagtttacg aaccgaacag gcttatgtca attcgcatga ccaaaatccc ttaacgtgag 3661 ttttcgttcc actgagcgtc agaccccgta gaaaagatcg caaccgcccg acggcgagga 3721 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt 3781 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt 3841 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct 3901 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt 3961 cttctgaccc ggggagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg 4021 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt 4081 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc 4141 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct 4201 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc 4261 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta 4321 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca 4381 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag 4441 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag 4501 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt 4561 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa 4621 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg 4681 attttggtca tgactagtgc ttggattctc accaataaaa aacgcccggc ggcaaccgag 4741 cgttctgaac aaatccagat ggagttctga ggtcattact ggatctatca acaggagtcc 4801 aagcgagctc gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct 4861 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta 4921 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcgg gacccacgct 4981 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg 5041 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa 5101 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt 5161 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta 5221 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca 5281 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta 5341 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct 5401 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg 5461 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac 5521 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact 5581 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa 5641 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactc //