LOCUS pJWT00001 5648 bp ds-DNA circular 23-DEC-2011 ACCESSION pJWT00001 VERSION pJWT00001 KEYWORDS . FEATURES Location/Qualifiers CDS 1..2 /label="AmpR" /vntifkey="4" /ApEinfo_fwdcolor="#00ffff" /ApEinfo_revcolor="#00ffff" misc_marker 138..434 /label="Kan/neoR 5prime" /vntifkey="22" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /note="encodes nptII (aka AphA, neoR), gives kan and neo resistance, with native promoter in on AatII and SpeI from Lutz vector pZE2PLtetO-1 MCS2" CDS 483..1565 /label="lacI" /vntifkey="4" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /note="lacI does not contain internal EcoRV site" promoter 1934..2009 /label="lacUV5 promoter" /vntifkey="30" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" misc_binding 1950..2013 /label="lac operator" /vntifkey="20" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" RBS 2029..2048 /label="RBS" /vntifkey="32" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" CDS 2049..2726 /label="RFP" /vntifkey="4" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" misc_marker 2881..3540 /label="CmR" /vntifkey="22" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" misc_marker 3667..3928 /label="Kan/neoR 3prime" /vntifkey="22" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /note="encodes nptII (aka AphA, neoR), gives kan and neo resistance, with native promoter in on AatII and SpeI from Lutz vector pZE2PLtetO-1 MCS2" rep_origin 3972..4654 /label="colE1" /vntifkey="33" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" terminator 4660..4765 /label="T0" /vntifkey="43" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" CDS 4795..5648 /label="AmpR" /vntifkey="4" /ApEinfo_fwdcolor="#00ffff" /ApEinfo_revcolor="#00ffff" primer_bind 4811..4830 /label="RDR00097" /ApEinfo_fwdcolor="#996633" /ApEinfo_revcolor="#996633" misc_binding 4930..4935 /label="BsaI_site" /ApEinfo_fwdcolor="#00ff00" /ApEinfo_revcolor="#00ff00" variation 4931 /label="AtoG_mutation" /ApEinfo_fwdcolor="#ff0000" /ApEinfo_revcolor="#ff0000" terminator 2751..2784 /label="Bba_1000_Term" ORIGIN 1 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga 61 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga 121 aaagtgccac ccccgggatg attgaacaag atggattgca cgcaggttct ccggccgctt 181 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg 241 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg 301 gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg 361 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg 421 gcgaagtgcc gggggacgtc ggtgcctaat gagtgagcta acttacatta attgcgttgc 481 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc 541 aacgcgcggg gagaggcggt ttgcgtattg ggcgccaggg tggtttttct tttcaccagt 601 gagacgggca acagctgatt gcccttcacc gcctggccct gagagagttg cagcaagcgg 661 tccacgctgg tttgccccag caggcgaaaa tcctgtttga tggtggttaa cggcgggata 721 taacatgagc tgtcttcggt atcgtcgtat cccactaccg agatgtccgc accaacgcgc 781 agcccggact cggtaatggc gcgcattgcg cccagcgcca tctgatcgtt ggcaaccagc 841 atcgcagtgg gaacgatgcc ctcattcagc atttgcatgg tttgttgaaa accggacatg 901 gcactccagt cgccttcccg ttccgctatc ggctgaattt gattgcgagt gagatattta 961 tgccagccag ccagacgcag acgcgccgag acagaactta atgggcccgc taacagcgcg 1021 atttgctggt gacccaatgc gaccagatgc tccacgccca gtcgcgtacc gtcttcatgg 1081 gagaaaataa tactgttgat gggtgtctgg tcagagacat caagaaataa cgccggaaca 1141 ttagtgcagg cagcttccac agcaatggca tcctggtcat ccagcggata gttaatgatc 1201 agcccactga cgcgttgcgc gagaagattg tgcaccgccg ctttacaggc ttcgacgccg 1261 cttcgttcta ccatcgacac caccacgctg gcacccagtt gatcggcgcg agatttaatc 1321 gccgcgacaa tttgcgacgg cgcgtgcagg gccagactgg aggtggcaac gccaatcagc 1381 aacgactgtt tgcccgccag ttgttgtgcc acgcggttgg gaatgtaatt cagctccgcc 1441 atcgccgctt ccactttttc ccgcgttttc gcagaaacgt ggctggcctg gttcaccacg 1501 cgggaaacgg tctgataaga gacaccggca tactctgcga catcgtataa cgttactggt 1561 ttcacattca ccaccctgaa ttgactctct tccgggcgct atcatgccat accgcgaaag 1621 gttttgcgcc attcgatggt gtccgggatc tcgacgctct cccttatgcg actcctgcat 1681 taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc 1741 atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc 1801 gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc 1861 gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc 1921 gtagaggatc gagatcgttt aggcacccca ggctttacac tttatgcttc cggctcgtat 1981 aatgtgtgga attgtgagcg gataacaatt tcagaattca aaagatcttt taagaaggag 2041 atatacatat ggcgagtagc gaagacgtta tcaaagagtt catgcgtttc aaagttcgta 2101 tggaaggttc cgttaacggt cacgagttcg aaatcgaagg tgaaggtgaa ggtcgtccgt 2161 acgaaggtac ccagaccgct aaactgaaag ttaccaaagg tggtccgctg ccgttcgctt 2221 gggacatcct gtccccgcag ttccagtacg gttccaaagc ttacgttaaa cacccggctg 2281 acatcccgga ctacctgaaa ctgtccttcc cggaaggttt caaatgggaa cgtgttatga 2341 acttcgaaga cggtggtgtt gttaccgtta cccaggactc ctccctgcaa gacggtgagt 2401 tcatctacaa agttaaactg cgtggtacca acttcccgtc cgacggtccg gttatgcaga 2461 aaaaaaccat gggttgggaa gcttccaccg aacgtatgta cccggaagac ggtgctctga 2521 aaggtgaaat caaaatgcgt ctgaaactga aagacggtgg tcactacgac gctgaagtta 2581 aaaccaccta catggctaaa aaaccggttc agctgccggg tgcttacaaa accgacatca 2641 aactggacat cacctcccac aacgaagact acaccatcgt tgaacagtac gaacgtgctg 2701 aaggtcgtca ctccaccggt gcttaaggat ccaaactcga gtaaggatct cgcaaaaaac 2761 cccgcttcgg cggggttttt tcgccctagg gcgttcggct gcggcgagcg gtatcagctc 2821 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt 2881 ttacgccccg ccctgccact catcgcagta ctgttgtaat tcattaagca ttctgccgac 2941 atggaagcca tcacaaacgg catgatgaac ctgaatcgcc agcggcatca gcaccttgtc 3001 gccttgcgta taatatttgc ccatggtgaa aacgggggcg aagaagttgt ccatattggc 3061 cacgtttaaa tcaaaactgg tgaaactcac ccagggattg gctgagacga aaaacatatt 3121 ctcaataaac cctttaggga aataggccag gttttcaccg taacacgcca catcttgcga 3181 atatatgtgt agaaactgcc ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt 3241 ttcagtttgc tcatggaaaa cggtgtaaca agggtgaaca ctatcccata tcaccagctc 3301 accgtctttc attgccatac gaaattccgg atgagcattc atcaggcggg caagaatgtg 3361 aataaaggcc ggataaaact tgtgcttatt tttctttacg gtctttaaaa aggccgtaat 3421 atccagctga acggtctggt tataggtaca ttgagcaact gactgaaatg cctcaaaatg 3481 ttctttacga tgccattggg atatatcaac ggtggtatat ccagtgattt ttttctccat 3541 tttagcttcc ttagctcctg aaaatctcga taactcaaaa aatacgcccg gtagtgatct 3601 tatttcatta tggtgaaagt tggaacctct tacgtgccga tcaacgtctc attttcgcca 3661 gatatcgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat 3721 catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga 3781 ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg 3841 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt 3901 ctatcgcctt cttgacgagt tcttctgacc cggggagcaa aaggccagca aaaggccagg 3961 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat 4021 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag 4081 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga 4141 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg 4201 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt 4261 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac 4321 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc 4381 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt 4441 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc 4501 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc 4561 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg 4621 aacgaaaact cacgttaagg gattttggtc atgactagtg cttggattct caccaataaa 4681 aaacgcccgg cggcaaccga gcgttctgaa caaatccaga tggagttctg aggtcattac 4741 tggatctatc aacaggagtc caagcgagct cgtaaacttg gtctgacagt taccaatgct 4801 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac 4861 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa 4921 tgataccgcg ggacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg 4981 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt 5041 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca 5101 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt 5161 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct 5221 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg 5281 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg 5341 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg 5401 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa 5461 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt 5521 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt 5581 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt 5641 gaatactc //